臨床生物化學(xué)實(shí)用教程

內(nèi)容概要

本書是一部注重理論和和實(shí)踐結(jié)合的臨床生化教程。作者認(rèn)為親自動(dòng)手去做實(shí)驗(yàn)會(huì)極大地增強(qiáng)人們對分析技術(shù)和所采用儀器的理解，因此，本書作者力求覆蓋在臨床生物化學(xué)中使用的主要分析技術(shù)和儀器，分析技術(shù)主要包括色譜學(xué)、光學(xué)、電化學(xué)、電泳、酶學(xué)、免疫學(xué)、分子診斷學(xué)和其它一些技術(shù)，并附以練習(xí)幫助學(xué)生對于儀器分析應(yīng)用的理解。要目：分光光度；X光檢測法；酶學(xué)；原子泄出和吸收；離子選電極；色譜法；薄層色譜法和提取技術(shù)；氣體色譜法；高性能液態(tài)色譜法；電泳法；分子診斷學(xué)；免疫學(xué)技術(shù)和X射線檢測法。讀者對象：臨床生物化學(xué)和相關(guān)專業(yè)的本科生及研究生。

書籍目錄

AcknowledgmentsAbbreviations.Chapter 1. Spectrophotometry  Photometric Analysis.    The Effect of Solvent 1 5  Laws of Radiant Energy Absorption.    Lambert's Law 1-6: Beer's Law 1-6; Beer Lambert's Law 1-8,    Interrelationship Between Absorbance and Transmittance I 8  Colour and Colorimeters.  The Blank.  Instrumentation.    Sources of Radiant Energy 1-9; Means of Spectral isolation 1 11;    Adjustable Diaphragm 1-15; Cuvettes 1-15; Radiant Energy Detectors 1-16; Readout Systems 1-21; A Typical Single Beam Speetrophotometer 1-21; Double Beam lnstruments 1-22; A Typical Double Beam up" Period 1 25; Optimum Spectral Band Width 1-25; Stray Light 1 27;Correction for BackRround Absorption 1-30   Checking Instrument Performance.    Wavelength Alignment I 33: Wavelength Repeatability 1-34; Spectral BandWidth 1-34; StrayLight 135:Pbotometric Aecuracy and Linearity 1-35  Exercises.    Exercise 1-1: Photometric Accuracy and Linearity.    Exercise 1-2: Spectral Band Width.    Exercise 1-3: Spectral Characteristics of a Filter.    Exercise 1-4: Wavelength Check.    Exercise 1-5: Spectral Band Width and Resolution.    Exercise 1-6: Cuvette Cut-Off Wavelengths.    Exercise 1-7: The Absorption Spectrum of Benzene.    Exercise 1-8: The Absorption Spectra of NAD and NADH,    Exercise 1-9: Stray Light.    Exercise 1-10: Derivative Spectra.    Exercise 1-11: The Determination of Serum Calcium.    Exercise 1-12: The Determination of Carbon Monoxide in Blood.    Exercise 1-13: The Determination of Plasma SaEey[ate.    Exercise 1-14: The Determination of Plasma ParacetamobChapter 2.  Fluorimetry  Fluorescence,    Factors Affecting Fluorescence Intensity : Concentration 2-3, Solvent 2 4,    Buffers 2-5,  pH and lonisation 2-5,  Temperature 2-6,  Photo-decomposition 2-6, Quenching 2-6 instrumentation,    Light Sources 2 8; Sample Containers 2-9; Detectors 2-9; Sensitivity 2-9; An Example of a Spectrofluorimeter 2 10  Exercises    Exercise 2-1 : The Effect of Concentration on Fluorescence Intensity.    Exercise 2-2 : ]he Effect of Solvent on Fluorescence Intensity.    Exercise 2-3 : The Effect of pH on Fluorescence Intensity.    Exercise 2-4 : The Effect of Temperature on Fluorescence Intensity.    Exercise 2-5 : The Effect of Quenching on Fluorescence intensity.    Exercise 2-6 : The Emission Spectrum of NADtt.    Exercise 2 7 : The Determination of Thiamine in Urine.    Exercise 2-8 : The Determination of Serum Magnesium.    Exercise 2 9 : The Determination of Phenylalanine in Plasma.    Exercise 2 10 : The Determination of Blood Porphyrins.    ReferencesChapter 3.Enzymology.  Enzymes.    Catalysis 3-1; Classiflcation and Nomenclature 3-2; EnzymeDnits 3 3:Modes of Action 3-4; The Active Site 3-4: Substrate Specificity 3-4; pH3-5; Temperature 3-5; Enzyme Kinetics 3-6; Enzyme Inhibitlon 3-9: Allosteric Enzymes 3-12; Isoenzymes 3-13  Clinical Enzymology.    The Diagnostic Use of lsoenzymes 3-17  Enzymes as Reagents.  Exercises.    Exercise 3-1: The Polarimetric Determhlatlon of tnvertase.    Exercise 3-2: The Effect of Temperature on lnvertase Activity.    Exercise 3-3: The Effect of pH on [nvertase Activity.    Exercise 3-4: The Effect of Substrate on Yeast Alcohol Dehydrogenase Activity,    Exercise 3-5 : The Effect of Substrate Concentration on Yeast Alcohol Dehydrogenase Activity.    Exercise 3-6 : The Effect of an Inhibitor on Yeast Alcohol Dehydrogenase Activity.    Exercise 3-7 : The Determination of Prostatic Acid Phosphatase in Plasma.    Exercise 3-8 : The Determination of Plasma Lactate Dehydrogenase and its lsoenzymes,    Exercise 3 9 : The Fluorimetric Determination of Alkaline Fhosphatase in Plasma.……Chapter4. Atomic Emission and AbsorptinChapter5. Ion Selective Electrodes.Chapter6. Oxygen and Carbon Dixide Electrodes.Chapter7. Chromatography.Chapter8. Thin Layer Chromatography and Extraction Techniques.Chapter9. Gas Chromatography.Chapter10. High Performance Liquid Chromatography.Chapter11. Electophoresis.Chapter12. Molecular Diagnostics.Chapter13. Immunological and Radioisotope Techniques.Chapter14. Coulometry,Osmometry and RefractometryAppendicesIndex

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