植物病害分子流行學(xué)導(dǎo)論

出版時間:2007-9  出版社:中國農(nóng)業(yè)大學(xué)出版社  作者:駱勇,馬忠華,馬占鴻 著  頁數(shù):208  字?jǐn)?shù):340000  

內(nèi)容概要

  《植物病害分子流行學(xué)導(dǎo)論(英文版)》主要內(nèi)容包括:Protein—based marker:Allozymes、DNA band—based markers、DNA sequence、References and suggested readings、CltAPTER 3 PCR—BASED TECHNIQUES、Classical PCR、Allele—specific PCR、Nested PCR、Multiplex PCR等等。

書籍目錄

PART I  INTRoDUCTION CHAPTER 1  INTRoDUCl’ION TO MoLECULAR EPIDEMIOLoGY  1—1 Basic concept of molecular epidemiology  1—2 Why molecular epidemiology  1—3 Comparisons between classical and molecular methods in epidemiology  1—4 Working approaches of molecular epidemiology  1—5 About the book  References and suggested readingsPARTⅡMoLECULAR TECHNIQUES USED IN EPIDEMIOLOGY CHAPTER 2 MOLECULAR MARKERS  2—1 Protein—based marker:Allozymes  2—2  DNA band—based markers  2—3  DNA sequence  References and suggested readings CltAPTER 3 PCR—BASED TECHNIQUES  3—1  Classical PCR  3—2  Allele—specific PCR  3—3  Nested PCR  3—4  Multiplex PCR  References and suggested readings CHAPTER 4 QUANTITATIVE REAL‘TIME PCR  4—1  Introduction  4—2  Basic real—time PCR methods  4—3 Primer and probe design for real—time PCR  4—4  Result analysis  4—5  Applications of real—time PCR  References and suggested readings CHAmR 5  PRIMER I)ESIGN  5—1  Some basic concepts for primer design  5—2 Computer programs used for primer design  5—3  An example of primer design using a computer program  5—4 Degenerated PCR primersPARTⅢ  MoLECULAR EPIDEMIOLOGY oF PLANT DISEASES CHAPTER 6 PATHoGEN IDENTIFICATIoN AND DISEASE DIAGNoSIS  6—1  Introduction  6—2 Use of species—specific genes or DNA regions for developing PCR primers  6—3 Use of anonymous unique DNA regions for developing PCR primers  6—4 PCR inhibitors  Summary  References and suggested readings CHAPTER 7 FUNGAL PATHoGEN REPRoDUCTIVE MoDES  7—1  Frequencies of genotypes  7—2 Deviation from Hardy—Weinberg expectations  7—3 Parsimony Tree Length Permutation(PTLPT)  7—4  Index of association(IA)  References and suggested readings CHAPTER 8  DETEMINATIoN AND QUANTIFICATION OF INITIAL INOCULUM OF EPIDEMICS  8—1  Introduction  8—2  Molecular methods for quantification of initial inoculum  8—3 Examples of quantification of initial inoculum using molecular methods  Remarkable conclusion  References and suggested readings CHPTER 9 TEMpORAL AND SPATIAL DEVELOPMENT oF PATHoGEN POPULATIoNS  9—1  Introduction  9—2  Molecular approaches  9—3  Monitoring pathogen population structures  9—4 Studying spatial—temporal dynamics of pathogen populations  9—5 Providing information needed for disease management  Summary  References and suggested readings CHAPTER 10  PATHoGEN LONG—DISTANCE DIsPERSAL AND MIGRATION  10—1  Introduction  10—2 Pathogen lon9—distance dispersal and disease epidemics  10—3 Traditional methods to determine or infer pathogen lon9—distance dispersal  10—4 Molecular methods to study pathogen lon9—distance dispersal  10一5  Examples  Summary  ……PART IV METHODOLOGY OF DATA ANLYSISINDEX

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