組織學(xué)技術(shù)

出版時間:2008-7  出版社:北京大學(xué)醫(yī)學(xué)出版社  作者:(英)班克羅夫特,等 編  頁數(shù):725  

前言

In the 30 years since the first edition of this book. histolechnology has continued to develop into a highly complex branch of laboratory medicine.Immunohistochemistry,in situ hybridization, molecular pathology,genetic testing and laser capture are all techniques currently in use to establish a diagnosis or to assess the changes occurring in tissues and cells in the disease process. Far more information can be obtained from these techniques than from many of the empirical methods used previously, but knowledge of the old and new is required by trained and trainee histolechnologists as well as the pathologist.The successful training of laborutory staff of all grades requires a thorough grounding in all aspects of histological techniques.In producing this edition, we continued to be faced with the problem of achieving a balance between the new and old technology. To help achieve this some chapters from the last edition have been amalgamated to alllow the introdution of the new material.There are a number of new chapters and contributors for this edition.The new chaoters include The gross room/surgical cutup by Paul Billings and William Grizzle,Tissue microarray by Wanda Grace-Jones,Genetic testing by Caroline Astbury,Laser microdissection by Diane Sterchi,and Jan Minshew has written Ergonomics.

內(nèi)容概要

《組織學(xué)技術(shù)(第6版)(英文影印版)》自1977年第一版問世以來,歷經(jīng)修訂,已成為全世界組織學(xué)家,病理學(xué)家的重要工具書?!督M織學(xué)技術(shù)(第6版)(英文影印版)》內(nèi)容全面,涵蓋了組織學(xué)技術(shù)的方方面面,既包括傳統(tǒng)的常用技術(shù),又介紹了近年來新發(fā)展的、有較高實用價值的新技術(shù)。各章節(jié)分別論述了光鏡、組織固定、樣本處理、冰凍切片、石蠟切片、酶組織學(xué)、免疫組化、免疫熒光、組織芯片、原位雜交、熒光原位雜交、激光顯微解剖、電鏡等技術(shù);對于結(jié)締組織、糖類,脂類、蛋白質(zhì).核酸、色素及礦物質(zhì)、淀粉、神經(jīng)內(nèi)分泌顆粒。微體、骨。神經(jīng)系統(tǒng)等相關(guān)的組織學(xué)技術(shù)也予專門章節(jié)講述;另外還涉及了顯微鏡樣品定量,實驗室安全及管理,以及人體工程學(xué)等相關(guān)內(nèi)容。既介紹各技術(shù)的理論、機制,又講述其應(yīng)用價值,特別是對有重要診斷意義的技術(shù)予以重點論述,凸現(xiàn)了組織學(xué)技術(shù)對臨床實踐的意義。

書籍目錄

List of contributorsPrefacePreface to the first editionGeneral acknowledgmentsAcknowIedgment to Alan Stevens1.Managing the LaboratoryMarilyn Gamble,lain Banks and John D.Bancroft2.Safety in the LaboratoryRichard W.Dapson3.Light MicroscopyJohn D.Bancroft and Alton D.Floyd4.Fixation of TissuesWilliam E.Grizzle,Jerry L.Fredenburgh and Russell B.Myers5.The Gross Room/Surgical CutupPaul E.Billings and William E.Grizzle6.Tissue ProcessingLenaT.Spencer and John D.Bancroft7.Microtomy:Paraffin and FrozenLena T.Spencer and John D.Bancroft8.How do Histological Stains Work?Richard W.Horobin9.The Hematoxy 8ins and EosinMarilyn Gamble10.Connective Tissues and StainsM.Lamar Jones,John D.Bancroft and Marilyn Gamble11.CarbohydratesRussell B.Myers,Jerry L.Fredenburgh and William E.Grizzle12.LipidsM.Lamar Jones13.Proteins and Nudeic AcidsJerry L.Fredenburgh,John D.Bancroft,William E.Grizzle and Russell B.Myers14.Pigments and MineralsCharies J.Churukian15.AmyloidGeoffrey H.Vowles16.The Dispersed Neuroendocrine System,Cytoplasmic Granules and other OrganellesWilliam E.Grizzle and John D.Bancroft17.MicroorganismsJeanine H.Bartlett18.BoneGayle M.Callis19.Techniques in NeuropathologyScott L.Nestor20.Enzyme Histachemistry and its Diagnostic ApplicationsScott L.Nestor and John D.Bancroft21.lmmunohistochemical TechniquesPeter Jackson and David Blythe22.lmmunohistochemistry Quality ControlChrista L.Hladik and Charles L.White23.lmmunohistochemistry Applications in PathologyCharles L.White24.lmmunofluorescent TechniquesChrista L.Hladik and Charles L.White25.Tissue MicroarrayWanda Grace-Jones26.Molecular Pathology——In Situ hybridizationDlane L.Sterchi27.Genetic Testing:Utilization of Fluorescent In Situ Hybridization(FISH)Caroline Astbury28.Laser MicodissectionDiane L.Sterchi29.Plastic Embedding for Light MicroscopyNeil M.Hand30.Electron MicroscopyAnthony E.Woods and John w.StMing31.Quantitative Data from Microscopic SpecimensAlton D.Floyd32.ErgonomicsJanet,MinshewAppendicesWilliam E.Grizzle,Jerry L.Fredenburgh and Russell B.MyersStaining Methods IndexSubject Index

章節(jié)摘錄

Medicine itself is a risky business,which requires careful clinical management.Histopathology is a significant aspect of the medical risk management process.The surgical biopsy is sent for histopathological assessment to corroborate or dispute the clinical diagnosis by providing confirmation of data provided from other diagnostic tests.It gives the clinician valuable information on how to proceed with the treatment of the disease.Major resections are referred to the laboratory to confirm the diagnosis,and in the case of malignant tumors to ensure that there are adequate resection margins,to determine the extent of lymphatic involvement and/or direct spread,and to stage and classify the disease.Autopsies provide definitive data for medical audit,and can be used to determine where medical procedures have been ineffective and also to give additional data for the future treatment of other patients with similar medical conditions.……

圖書封面

評論、評分、閱讀與下載


    組織學(xué)技術(shù) PDF格式下載


用戶評論 (總計0條)

 
 

 

250萬本中文圖書簡介、評論、評分,PDF格式免費下載。 第一圖書網(wǎng) 手機版

京ICP備13047387號-7