元基因組學(xué)

出版時(shí)間:2012-3  出版社:科學(xué)出版社  作者:(德)斯特賴特,(德)丹尼爾 著  頁數(shù):341  

內(nèi)容概要

  《元基因組學(xué):方法和步驟(影印版)》涵蓋了元基因組文庫的構(gòu)建、元基因組文庫的篩選技術(shù)和針對(duì)不同活性目標(biāo)產(chǎn)物的篩選方法,并且提供了詳細(xì)實(shí)用的實(shí)驗(yàn)操作方法,可以幫助希望從事元基因組學(xué)研究的科技人員盡快建立相關(guān)的實(shí)驗(yàn)平臺(tái)。
  《元基因組學(xué):方法和步驟(影印版)》適合于從事環(huán)境微生物學(xué)、微生物生態(tài)學(xué)等相關(guān)專業(yè)的高年級(jí)本科生、研究生,以及相關(guān)研究工作者等參考使用。

書籍目錄

Preface
Contributors
Molecular Methods to Study Complex Microbial Communities
Construction of Small-Insert and Large-Insert Metagenomic
Libraries
Construction and Screening of Marine Metagenomic Libraries
Metagenomic Analysis of Isotopically Enriched DNA
Wide Host-Range Cloning for Functional Metagenomics
Cloning and Expression Vectors for a Gram-Positive Host,
Streptomyces lividans
Heterologeus Gene Expression in the Hyperthermophilic Archaeon
Sulfolobus solfataricus
Novel Tools for the Functional Expression of Metagenomic DNA
Screening of Functional Promoter from Metagenomic
DNA for Practical Use in Expression Systems
Substrate-Induced Gene Expression Screening: A Method
for High-Throughput Screening of Metagenome Libraries
Screens for Active and Stereoselective Hydrolytic Enzymes
Screening for Cellulase-Encoding Clones in Metagenomic
Libraries
Screening Metagenomic Libraries for Laccase Activities
Screening for N-AHSL-Based-Signaling Interfering Enzymes
Identification of Molecular Markers to Follow Up the
Bioremediation
of Sites Contaminated with Chlorinated Compounds
Methods for the Isolation of Genes Encoding Novel PHB
Cycle Enzymes from Complex Microbial Communities
Metagenomic Approaches to Identify and Isolate Bioactive
Natural Products from Microbiota of Marine Sponges
Screening for Novel Antibiotic Resistance Genes
Novel Metal Resistance Genes from Microorganisms:A Functional
Metagenomic Approach
Retrieval of Full-Length Functional Genes Using Subtractive
Hybridization Magnetic Bead Capture
Detection and Isolation of Selected Genes of Interest from
Metagenomic Libraries by a DNA Microarray Approach
Application ofDNA Microarray for Screening Metagenome Library
Clones
MetaGenomeThreader:. A Software Tool for Predicting
Genes in DNA-Sequences of Metagenome Projects
Index

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