從基因到基因組

內容概要

基因技術、遺傳工程、重組DNA和基因克隆等技術的快速發(fā)展，把分子生物學推到了生命科學的前沿。本書對分子生物學核心技術的原理及其使用進行了簡明扼要的闡釋。以對分子生物學的基本概念的簡短介紹開篇，然后介紹核心的分子生物學方法及其整合運用，從單個基因的克隆與研究到整個基因組的測序，以及基因組信息的分析。最后，本書還介紹了這些技術在生物技術、醫(yī)藥和農業(yè)以及整個生命科學研究中的應用。    本書內容涵蓋了分子生物學基礎、基因克隆、核酸的純化與分離、DNA的剪切與連接、載體、基因組文庫和cDNA文庫、聚合酶鏈反應、DNA測序、序列數(shù)據(jù)分析、基因變異分析、基因表達分析、基因功能分析、醫(yī)藥應用、轉基因等等方面。    本書適于生物化學、分子生物學、遺傳學、細胞生物學、生物技術、生物工程等相關學科及研究領域的本科生、研究生及教學科研人員參考使用。

書籍目錄

Preface1 Introduction2 Basic Molecular Biology　2.1 Nucleic Acid Structure　2.2 Gene Structure and Organization　2.3 Information Flow:Gene Expression3 How to Clone a Gene　3.1 What is Cloning?　3.2 Wverview of the Procedures　3.3 Gene Libraries　3.4 Hybridization　3.5 Polymerase Chain Reaction4 Purification and Separation of Nucleic Acids　4.1 Extraction and Purification of Nucleic Acids　4.2 Detection and Quantitation of Nucleic Acids　4.3 Gel Electrophoresis5 Cutting and Joining DNA　5.1 Restriction Endonucleases　5.2 Ligation　5.3 Alkaline Digests　5.4 Double Digests　5.5 Modification of Restriction Fragment Ends　5.6 Other Ways of Joining DNA Molecules　5.7 Summary6 Vectors　6.1 Plasmid Vectors　6.2 Vectors Based on the Lambda Bacteriophage　6.3 Cosmids　6.4 M13 Vectors　6.5 Expression Vectors　6.6 Vectors for Cloning and Expression in Eukaryotic Cells　6.7 Supervectors:YACs and BACs　6.8 Summary7 Genomic and cDNA Libraries　7.1 Genomic Libraries　7.2 Growing and Storing Libraries　7.3 cDNA Libraries　7.4 Random,Arrayed and Ordered Libraries8 Finding the Right Clone　8.1 Screening Libraries with Gene Probes　8.2 Screening Expression Libraries with Antibodies　8.3 Rescreening　8.4 Subcloning　8.5 Characterization of Plasmid Clones9 Polymerase Chain Reaction(PCR)　9.1 The PCR Reaction　9.2 PCR in Practice　9.3 Cloning PCR Products　9.4 Long-range PCR　9.5 Reverse-transcription PCR　9.6 Rapid Amplification of cDNA Ends(RACE)　9.7 Applications of PCR10 DNA Sequencing　10.1 Principles of DNA Sequencing　10.2 Automated Sequencing　10.3 Extending the Sequence　10.4 Shotgun Sequencing:Contig Assembly　10.5 Genome Sequencing11 Analysis of Sequence Data　11.1 Analysis and Annotation　11.2 Databanks　11.3 Sequence Comparisons12 Analysis of Genetic Variation　12.1 Nature of Genetic Variation　12.2 Methods for Studying Variation13 Analysis of Gene Expression　13.1 Analysing Transcription　13.2 Comparing Transcriptomes　13.3 Methods for Studying the Promoter　13.4 Translational Analysis14 Analysis of Gene Function　14.1 Relating Genes and Functions　14.2 Genetic Maps　14.3 Relating Genetic and Physical Maps　14.4 Linkage Analysis　14.5 Transposon Mutagenesis　14.6 Allelic Replacement and Gene Knock-outs　14.7 Complementation　14.8 Studying Gene Function through Protein Interactions15 Manipulating Gene Expression　15.1 Factors Affecting Expression of Clonde Genes　15.2 Expression of Cloned Genes in Bacteria　15.3 Expression in Eukaryotic Host Cells　15.4 Adding Tags and Signals　15.5 In vitro Mutagenesis16 Medical Applications,Present and Future　16.1 Vaccines　16.2 Detection and Identification of Pathogens　16.3 Human Genetic Diseases17 Transgenics　17.1 Transgenesis and Cloning　17.2 Animal Transgenesis and its Applications　17.3 Transgenic Plants and their Applications　17.4 SummaryBibliographyGlossaryIndex

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