果蠅實(shí)驗(yàn)指南

內(nèi)容概要

果蠅是遺傳學(xué)、發(fā)育學(xué)研究的重要模式生物之一，對(duì)于發(fā)育生物學(xué)、基因組學(xué)領(lǐng)域中的探索具有極為重要的意義。本書是冷臬港實(shí)驗(yàn)室出版社Drosophila Protocols的影印版，共提供了近40個(gè)未來(lái)10年內(nèi)最可能用到的以果蠅為實(shí)驗(yàn)對(duì)象的分子生物學(xué)、生物化學(xué)和細(xì)胞生物學(xué)研究的實(shí)驗(yàn)方案。每一個(gè)方案都經(jīng)過(guò)專家的精心挑選和雕琢，實(shí)驗(yàn)設(shè)計(jì)嚴(yán)謹(jǐn)、準(zhǔn)確、簡(jiǎn)潔、規(guī)范，可操作性強(qiáng)，值得稱道。方案涉及染色體、細(xì)胞、基因組和發(fā)育等方面，既包含了初學(xué)者需要了解的基礎(chǔ)知識(shí)，也涵蓋了資深研究者所需的細(xì)節(jié)。本書的版式設(shè)計(jì)側(cè)重于方便讀者使用，正文中穿插了豐富的圖表作為實(shí)驗(yàn)設(shè)計(jì)的輔助說(shuō)明，附錄中還列出了果蠅研究所需的重要資料如基本溶液、緩沖液的配方和配制方法等。    本書適合于從事發(fā)育生物學(xué)、細(xì)胞生物學(xué)、基因組學(xué)、遺傳學(xué)、分子生物學(xué)、藥物設(shè)計(jì)和開發(fā)以及功能基因?qū)W組研究的相關(guān)教學(xué)科研人員以及相關(guān)學(xué)科的本科生、研究生參考使用。

書籍目錄

PrefaceAbbreviationsCHROMOSOMES　1 Preparation and Analysis of Drosophila Mitotic Chromosomes　　2 In Situ Hybridization to Somatic Chromosomes  3 BrdU Labeling of Chromosomes  4 Analysis of Meiosis of Fixed and Live Oocytes by Light Microscopy  5 Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster  6 Preparation and Analysis of Polytene Chromosomes  7 In Situ Hybridizaiton to Polytene Chromosomes  8 Mapping Protein Distributions on Polytene Chromosomes by ImmunostainingCELL BIOLOGY  9 Fluorescent Analysis of Drosophila Embryos  10 Imaginal Discs  11 The Neuromuscular Junction  12 Histological Techniques for the Drosophila Eye.Part Ⅰ:Larva and Pupa  13 Histological Techniques for the Drosophila Eye.Part Ⅱ:Adult  14 Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy  15 Electrophysiological Approaches to the Neuromusculature  16 Functional Assays of the Peripheral and Central Nervous Systems  17 GFP and Other Reporters  18 Quantitative Microinjection of Drosophila Embryos  19 Targeted Disruption of Gene Function in Drosophila by RNA Interference  20 Drosophila Cell Culture and TRransformation  21 Generating Antibodies against Drosophila Proteins  22 Photoactivated Gene Expression for Cell-fate Mapping and Cell ManipulationMOLECULAR BIOLOGY  23 Recovery of DNA Sequences Flanking P-element Insertions:Inverse PCR and Plasmid Rescue  24 GAL4-mediated Ectopic Gene Expression in Drosophila  25 Functional Cell AblationGENOMICS  26 Preparation of DNA from E.coli Cells Containing Bacteriophage P1 Clones  27 Drosophila Microarrays:From Arrayer Construction to Hybridization  28 Using Drosophila Genome Databases  29 Preparation and Analysis of High-molecular-weight DNA in DrosophilaBIOCHEMISTRY  30 Culturing Large Populations of Drosophila for Protein Biochemistry  31 Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis  32 Preparation of Membrane Proteins from Drosophila Embryos  33 Preparing Cytoplasmic Extracts from Drosophila Embryos  34 Immunoblotting of Proteins from Single Drosophila EmbryosTHE ORGANISM  35 Laboratory Culture of Drosophila  36 Preparation of Larval and Adult Cuticles for Light Microscopy  37 Exposing Drosophila to Neuroactive DrugsAPPENDICES  1 1989 Table of Contents from Drosophila:A Laboratory Manual  2 Anatomical Drawings of Drosophila  3 Solutions and Reagents  4 Cautions  5 Suppliers  6 TrademarksIndex

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